Vocabulary
| Supercoiling | DNA gyrase | Topoisomerase | Nuclear matrix proteins |
| Semiconservative | Replication fork | Okazaki fragment | Helicase |
| Primase | Nick translation | Ligase | DNA polymerase I |
| Transcription start site | Sense strand | Template strand | Promoter |
| Shine-Dalgarno | Aminoacyl tRNA synthetases | Peptidyl transferase | Kozak sequence |
| Wobble | Release factors | Charged tRNA | Polycistronic |
| diauxie | repressor | inducer | gratuitous inducer |
| operator | PaJaMo | trans-acting/cis-dominant | Co-repressor |
| lac promoter | cAMP-CRP | Constitutive | Attenuation |
How do the Shine-Dalgarno sequence and the 30S ribosome function coordinately in translation?
What is an open reading frame?
Distinguish between positive and negative modes of transcriptional regulation. How is it that negative regulation can be associated with "induction", while positive regulatory mechanisms can account for "repression"?
What evidence led to the proposal of the operon model by Jacob and Monod?