Study Guide to Exam III, Microbial Genetics
Vocabulary
| Lederberg and Zinder | Hershey-Chase | Transducing particle | stable transductant |
| abortive transductant | temperate phage | virulent phage | lysogeny |
| Lysogenic conversion | cI, cro, cII | Prophage | integrase |
| BOB and POP | Induction | xis (excisionase) | l dgal |
| stable transductant | adhesion zones | Competence complexes | Chi sites |
| artificial transformation-e.g. with cold Ca2+ | interrupted mating | Aberrant excision | Competence |
| Hfr | Electroporation | transfer replication (oriT) | Holliday junction |
| dam methylase | RecA | Lytic cycle | quorum sensing |
| MutHLS | Photolyase | Mismatch repair | RecBCD |
Questions and problems.
In what way did the Avery, MacCleod, McCarty and the Hershey-Chase experiments prove that DNA is the genetic material?
How does the mismatch repair system distinguish parental from newly replicated DNA?
How does RecA function in the induction of the lambda prophage?
Why is a specialized transducing phage with the E. coli gal marker defective, while the phage carrying the bio marker is not?
Be able to …
…present a generalized scheme for the uptake of naked DNA by gram-positive bacteria.
…describe the prototrophic recovery experiments of Tatum and Lederburg. What was the role of the U-tube (invented by Bernard Davis) in the characterization of this phenomenon?
…explain the difference between F+, F-, Hfr, and F'
...calculate frequency of transduction of a particular gene, given genome sizes (phage and host) and proportion of phage particles that are transducing.