Replicon |
Copy number |
Par |
Hok/Sok |
Incompatibility |
Curing |
Host restriction phenomenon |
hsdMRS |
Arber |
Smith/Nathans |
restriction endonuclease |
HindII |
Type II restriction endonucleases |
EcoRI |
sticky ends |
physical mapping |
Cohen and Boyer |
Berg/homopolymeric tailing |
cloning vectors |
blue-white selection |
polylinker (multiple cloning site) |
genomic DNA library |
insertional inactivation |
functional complementation |
colony hybridization |
plaque hybridization |
Southern blot (Northern, Western, etc) |
immunological screening |
A circular DNA molecule is digested with EcoR1, HindIII, PstI, and three double digests. Fragment sizes are as follows: EcoR1, 8 kb; HindIII, 7 kb and 1 kb; EcoR1/HindIII, 5kb, 2 kb, and 1 kb; Pst, 8 kb; EcoR1/Pst, 6 kb and 2 kb; Pst/HindIII, 4 kb, 3 kb, and 1 kb. Map the sites.
What are the general steps involved in cloning a gene? What are some methods for identifying a particular clone?
Which of the following are more likely to be a Type II RE recognition site? (only one strand is shown)
GAATCG, GATATC, AAATTT, ACGGCA