BIOL 4233 Microbial Genetics
MWF
www.uark.edu/campus-resources/mivey/m4233/
|
Instructor: |
Mack IveySCEN 627 (office) or FERR 315/316 (lab) Phone: 575-2729 (office) or 575-2416 (lab)Email: mivey@uark.edu |
|
Office hours: |
Monday and Wednesday 8-10 am, Tuesday 4-5 pm. I’m available at other times by appointment (email me). |
|
Prerequisites: |
General Microbiology (BIOL 2013/2011L) Organic Chemistry (CHEM 3603/3621 and CHEM 3613/3631) |
|
Text: |
Molecular Biology, David P. Clark; Elsevier, 2005 |
|
Exams and Grading: |
There will be four 50-minute exams and a 2-hour final
exam. The 50-minute exams will be worth 75% of the final grade.
Each will cover only new material, i.e., they will not be
comprehensive. Each will be closed book. Old exams are posted on the
class web site (http://www.uark.edu/campus-resources/mivey/m4233/exams2k.html). The final exam is optional. It will be comprehensive, but you will be allowed to bring your notebook and one textbook to the final exam. If you choose to take it, it will be worth 25% of your grade. I will calculate both the 4-exam average and the 5-exam average, and give you the higher of the two. In other words, it can’t hurt you to take the final. |
|
Make-ups: |
No exam can be dropped. Makeup exams will be permitted only if they are arranged one week (or more) prior to the scheduled exam date. You may make up only one missed exam. |
|
Cancellation Policy: |
I will leave a voice mail message at 575-2729, advising you of the status of class on snowy or icy days. |
|
Objective: |
To emphasize experimental approaches and problem solving in describing the principles of microbial (primarily bacterial and bacteriophage) genetics, so that you will understand modern genetic tools and be able to apply them. |
Tentative Schedule:
Aug 2006
|
Mo21 |
Intro to course, classical (i.e. non-molecular) genetics, alleles, genetic notation. Read Chapters 1 and 2. |
|
We23 |
Introduction to mutational analysis. |
|
Fr25 |
Mutational analysis, cont. |
|
Mo28 |
DNA
composition and structure. Chapter 3. |
|
We30 |
DNA
composition and structure. |
Sep 2006
|
Fri1 |
Higher order structure – Chromosomes in bacteria and eukaryotes. |
|
Mo4 |
Labor
Day |
|
We6 |
Chromosome structure. |
|
Fr8 |
Replication. Chapter 5. |
|
Mo11 |
Replication. |
|
We13 |
Replication. |
|
Fr15 |
Exam I |
|
Mo18 |
Introduction to gene expression, transcription. Chapters 6 and 12. |
|
We20 |
Transcription. |
|
Fr22 |
Transcription and Translation. Chapter 8 |
|
Mo25 |
Translation. |
|
We27 |
Regulation of gene expression in prokaryotes. Chapter 9 |
|
Fr29 |
Regulation of prokaryotic genes. |
Oct 2006
|
Mo2 |
Regulation in prokaryotes. |
|
We4 |
Regulation in eukaryotes. Chapters 10 and 11. |
|
Fr6 |
Regulation in eukaryotes. |
|
Mo9 |
Mutations. Chapter 13. |
|
We11 |
Exam II |
|
Fr13 |
Alternative splicing, etc. |
|
Mo16 |
RNAi |
|
We18 |
RNAi and Mutations |
|
Fr20 |
|
|
Mo23 |
Error repair. Chapter 14. |
|
We25 |
Error repair |
|
Fri27 |
Homologous recombination. Right-click here for mp3 of homologous recombination lecture |
|
|
October 27th is the last day to drop the
course |
|
Mo30 |
Genetic
transfer: conjugation. |
Nov 2006
|
We1 |
Genetic transfer: conj II and transduction. mp3 |
|
Fr3 |
Genetic transfer: transformation mp3 |
|
Mo6 |
Review Session mp3 |
|
We8 |
Exam
III |
|
Fr10 |
Transposition. Chapter 15. mp3 |
|
Mo13 |
Transposons and Lysogeny mp3 |
|
We15 |
Specialized transduction and Plasmids. Chapter 16. mp3 |
|
Fr17 |
Restriction endonucleases mp3 |
|
Mo20 |
Introduction to gene cloning. Chapters 21 and 22 mp3 |
|
We22 |
Fall Break |
|
Fr24 |
Thanksgiving
|
|
Mo27 |
Cloning mp3 |
|
We29 |
Cloning mp3 |
Dec 2006
|
Fr1 |
Review session |
|
Mo4 |
Exam IV |
|
|
|
|
Dead Day |
|
|
|
|
|
Final
Exam 10 am- |