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MBIO 4233 Microbial Genetics
Safari No. 02508, MWF 1:30-2:20, SCIE 218
http://www.uark.edu/campus-resources/mivey/m4233/
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Instructor: |
D Mack IveySCIE 415A (office) or SCIE 417 (lab) Phone: 575-2729Email: mivey@comp.uark.edu |
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Office hours: |
Monday and Wednesday 2:30–4:30 pm. I’m available at other times by appointment, or try dropping by SCIE 417 to catch me in the lab. |
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Prerequisites: |
General Microbiology (MBIO 2013/2011L) Organic Chemistry (CHEM 3603/3621 and CHEM 3613/3631) |
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Text: |
Genetics – Analysis and Principles, Robert J. Brooker; Benjamin Cummings, 1999 |
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Exams and Grading: |
There will be four 50-minute exams and a 2-hour final exam. The 50-minute exams will be worth 80% of the final grade. They will cover only new material, i.e., they will not be comprehensive. They will be closed book. The final exam, worth 20% of your grade, will be comprehensive, but you will be allowed to bring your notebook and one textbook to the final exam. |
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Make-ups: |
No exam can be dropped. Makeup exams will be permitted only if they are arranged one week (or more) prior to the scheduled exam date. You may make up only one missed exam. |
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Cancellation Policy: |
I will leave a voice mail message at 575-2729, advising you of the status of class on snowy or icy days. |
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Objective: |
To emphasize experimental approaches and problem solving in describing the principles of microbial (primarily bacterial and bacteriophage) genetics, so that you will understand modern genetic tools and be able to apply them. |
Tentative Schedule:
Day Aug 1999
1) Mo23 Intro to course, classical (i.e. non-molecular) genetics, alleles, genetic notation. “Gene” as an abstraction. Read Chapter 1 (all), and Chapter 2, pp 17-29, along with Chapter 3, pp 45-59. If you prefer, read the corresponding sections in a general biology textbook, instead.
2) We25 Introduction to mutational analysis. Read Chapter 4 (pp75-79), Chapter 5 (pp100-105, 111-119).
3) Fr27 Mutational analysis; Genetic transfer in bacteria (or, the application of “classical” genetics to bacteria). Read all of Chapter 6.
4) Mo30 Genetic transfer: conjugation.
Sep 1999
5) We1 Genetic transfer: transduction
6) Fri3 Genetic transfer: transformation.
Mo6 Labor Day
7) We8 DNA composition and structure.
8) Fr10 DNA molecular structure, part 2.
9) Mo13 Higher order structure – Chromosomes in bacteria and eukaryotes.
10) We15 Chromosome structure.
11) Fr17 Exam I
12) Mo20 Replication.
13) We22 Replication.
14) Fr24 Replication.
15) Mo27 Introduction to gene expression. The Central Dogma revisited, and the necessity for regulation.
16) We29 Transcription and the genetic code.
Oct 1999
17) Fr1 Transcription and Translation.
18) Mo4 Translation. Regulation in prokaryotes.
19) We6 Regulation of gene expression in prokaryotes. The operon concept, PaJaMo, etc.
20) Fr8 Regulation of prokaryotic genes
21) Mo11 Regulation in prokaryotes.
22) We13 Exam II
23) Fr15 Regulation in eukaryotes.
24) Mo18 Regulation in eukaryotes.
25) We20 Mutations.
26) Fr22 Mutations.
27) Mo25 Error repair.
28) We27 Error repair.
29) Fr29 Transposition.
******Last Day to Drop Course******
Nov 1999
30) Mo1 Introduction to gene cloning.
31) We3 Restriction/modification.
32) Fr5 Restriction endonucleases.
33) Mo8 Exam III
34) We10 Other enzymes, cloning.
35) Fr12 Recombinant DNA applications.
36) Mo15 Cloning, part II.
37) We17 Genome analysis.
38) Fr19 Genome analysis II.
39) Mo22 Biotechnology.
40) We24 Biotechnology.
Fr26 Thanksgiving Holiday
41) Mo29 Biotechnology.
Dec 1999
42) We1 Genetic basis of cancer.
43) Fr3 Genetic basis of cancer, part 2.
44) Mo6 Exam IV
We8 Dead Day
Saturday Dec 11 Final Exam 12:30 pm-2:30 pm